Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...

The gold standard for gene-expression analysis, qPCR, has been extensively studied. Standard protocols have been published and multiple instruments for cycling with real-time detection are ...

Novel techniques, automated machines, sophisticated synthetic and bioprospected polymerases, and developments in symbiotic sequencing technologies have transformed PCR into a multifaceted, adaptable ...

A new approach by LMU chemists could help to significantly improve diagnostic tests based on PCR. The enzymes used are triggered by light pulses. DNA polymerases and other enzymes that modify DNA are ...