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SOLVED: 7. Quantification of DNA can be done by using aNanodrop, a UV spectrophotometer, by measuring its absorbance inunits of optical density (OD) (see “Nanodrop MicrovolumeQuantitation of Nucleic Acids” video in Lab Activity Videos for Lab3 on Laulima for more information). DNA absorbs light most stronglyat the ultraviolet wavelength of 260 nm. The absorbance ofdouble stranded DNA (dsDNA) at 260 nm(A260) is used toestimate concentration, with 1.0 OD equal to a dsDNA concentrationof 50 μg/ml
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A spectrophotometer measures how much light a sample absorbs at specific wavelengths, based on Beer’s Law, for analyzing concentration. It uses components like a light source, monochromator, cuvette, and detector to separate, select, and detect light passing through the sample. Applications include chemistry, biology, medicine, material science, food, and pharma for purposes like testing reactions, quantifying DNA/proteins, and analyzing materials. #fyp #fypシ #bhfyp #fypage #fypシ❤️💞❤️ #fypシ゚vir
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